Education

Biography

Dr. Virginia Tirino is a Professor at University of Campania "L. Vanvitelli"in the department of Experimental Medicine - Section of Histology. She received Her PhD from Second University of Naples and degree from University of Naples. She has published 51 articles in the international publications.

Research Interest

• Dental Pulp Stem Cells
• Oral Cancer
• Bone Grafts
• Cell Culture
• Biomaterials
• Mesenchymal Stem Cells
• Cancer Stem Cells

Scientific Activities

Employment History

Professional Activities

Scientific Societies

Mesenchymal Stem cells differentiation into bone and Cancer Stem cells of solid tumours. Cell cultures and establishment of melanoma, sarcoma and lung primary cell lines and their subsequent phenotypic characterization by flow cytometry. Isolation and characterization of cancer stem cells from breast, lung, gastric and sarcomas using flow cytometry and electronic and magnetic cell sorter and put into culture of the same. Isolation and characterization of stem cells from dental pulp,adipose tissue, umbilicalcord and amniotic fluid by flow cytometry and cell sorter and put into culture of the same. Expertise in cell biology, flow cytometry and cell sorting.

Referee Activities

International Journals: Plos One, Tissue Engineering, Journal of Cellular Physiology, Journal of Cellular Biochemistry e Stem Cell Research and Therapy

Editorial Board

Journal of Stem cells Research, Reviews and Reports dal 2014

Society member

Member of Italian Society of Histology and Embryology since 2012 and Italian Society of Cytometry since 2004.

Honors and Awards

• Award of study GIC “La citometria in lavori originali inediti”, 22nd Edition 2008 , Urbino ,
• October 3, 2008
• Honorary Diploma for the PhD thesis received from ' Academy Ercolanese , February 3, 2012
• Prize of € 1,000 for the best oral communication presented at the 68th Congress of Human Anatomy and Histology, 18-20 September 2014, Ancona
• Prize of € 4,000 to the University Research from 2013 to 2014 at the Second University of Naples

Publications

1. Paino F, La Noce M, Giuliani A, De Rosa A, Mazzoni S, Laino L, Amler E, Papaccio G, Desiderio V, Tirino V. Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering. Clin Sci (Lond). 2017 Apr 25;131(8):699-713. doi: 10.1042/CS20170047.
2. Paino F, La Noce M, Di Nucci D, Nicoletti GF, Salzillo R, De Rosa A, Ferraro GA, Papaccio G, Desiderio V, Tirino V. Human adipose stem cell differentiation is highly affected by cancer cells both in vitro and in vivo: implication for autologous fat grafting. Cell Death Dis. 2017 Jan 19;8(1):e2568. doi: 10.1038/cddis.2016.308.
3. Stellavato A, Tirino V, de Novellis F, Della Vecchia A, Cinquegrani F, De Rosa M, Papaccio G, Schiraldi C. Biotechnological Chondroitin a Novel Glycosamminoglycan With Remarkable Biological Function on Human Primary Chondrocytes. J Cell Biochem. 2016 Sep;117(9):2158-69. doi: 10.1002/jcb.25556. Epub 2016 May 11.
4. Mele L, Vitiello PP, Tirino V, Paino F, De Rosa A, Liccardo D, Papaccio G, Desiderio V.Changing Paradigms in Cranio-Facial Regeneration: Current and New Strategies for the Activation of Endogenous Stem Cells. Front Physiol. 2016 Feb 24;7:62. doi:10.3389/fphys.2016.00062.
5. Perillo E, Porto S, Falanga A, Zappavigna S, Stiuso P, Tirino V, Desiderio V, Papaccio G, Galdiero M, Giordano A, Galdiero S, Caraglia M. Liposome armed with herpes virusderived gH625 peptide to overcome doxorubicin resistance in lung adenocarcinoma cell lines. Oncotarget. 2016 Jan 26;7(4):4077-92. doi: 10.18632/oncotarget.6013.
6. Pepe P, Castellano M, Alfano S, Della Pepa ME, Tirino V, Piemonte M, Desiderio V, Zappavigna S, Galdiero M, Caraglia M, Cringoli G, Rinaldi L, Galdiero M. Dicrocoelium dendriticum induces autophagic vacuoles accumulation in human hepatocarcinoma cells.Vet Parasitol. 2015 Sep 15;212(3-4):175-80. doi: 10.1016/j.vetpar.2015.07.039.
7. D'Agostino A, Stellavato A, Busico T, Papa A, Tirino V, Papaccio G, La Gatta A, De Rosa M, Schiraldi C. In vitro analysis of the effects on wound healing of high- and low-molecular weight chains of hyaluronan and their hybrid H-HA/L-HA complexes. BMC Cell Biol. 2015 Jul 11;16:19. doi: 10.1186/s12860-015-0064-6.
8. Cioffi M, D'Alterio C, Camerlingo R, Tirino V, Consales C, Riccio A, Ierano C, Cecere SC, Losito NS, Greggi S, Pignata S, Pirozzi G, Scala S. Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer. Sci Rep. 2015 May 28;5:10357. doi:10.1038/srep10357.
9. Lamberti M, Porto S, Zappavigna S, Stiuso P, Tirino V, Desiderio V, Mele L, Caraglia M.Levofolene modulates apoptosis induced by 5-fluorouracil through autophagy inhibition: clinical and occupational implications. Int J Oncol. 2015 May;46(5):1893-900. doi:10.3892/ijo.2015.2904.
10. Naddeo P, Laino L, La Noce M, Piattelli A, De Rosa A, Iezzi G, Laino G, Paino F, Papaccio G, Tirino V. Surface biocompatibility of differently textured titanium implants with mesenchymal stem cells. Den Mater. 2015 Mar;31(3):235-43. doi: 10.1016/j.dental.2014.12.015. Epub 2015 Jan 10.
11. Desiderio V, Papagerakis P, Tirino V, Zheng L, Matossian M, Prince ME, Paino F, Mele L, Papaccio F, Montella R, Papaccio G, Papagerakis S. Increased fucosylation has a pivotal role in invasive and metastatic properties of head and neck cancer stem cells. Oncotarget. 2015 Jan 1;6(1):71-84.
12. La Noce M, Mele L, Tirino V, Paino F, De Rosa A, Naddeo P, Papagerakis P, Papaccio G, Desiderio V. Neural crest stem cell population in craniomaxillofacial development and tissue repair. Eur Cell Mater. 2014 Oct 28;28:348-57.
13. Desiderio V, Tirino V, Papaccio G, Paino F. Bone defects: molecular and cellular therapeutic targets. Int J Biochem Cell Biol. 2014 Jun;51:75-8. doi: 10.1016/j.biocel.2014.03.025.
14. La Noce M, Paino F, Spina A, Naddeo P, Montella R, Desiderio V, De Rosa A, Papaccio G, Tirino V, Laino L. Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. J Dent. 2014 Jul;42(7):761-8. doi: 10.1016/j.jdent.2014.02.018.
15. Paino F, La Noce M, Tirino V, Naddeo P, Desiderio V, Pirozzi G, De Rosa A, Laino L Altucci L, Papaccio G. Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement. Stem Cells. 2014 Jan;32(1):279-89. doi: 10.1002/stem.1544.
16. Tirino V, Camerlingo R, Bifulco K, Irollo E, Montella R, Paino F, Sessa G, Carriero MV, Normanno N, Rocco G, Pirozzi G. TGF-β1 exposure induces epithelial to mesenchymal transition both in CSCs and non-CSCs of the A549 cell line, leading to an increase of migration ability in the CD133+ A549 cell fraction. Cell Death Dis. 2013 May 2;4:e620. doi:10.1038/cddis.2013.144.
17. Giuliani A, Manescu A, Langer M, Rustichelli F, Desiderio V, Paino F, De Rosa A, Laino L, d'Aquino R, Tirino V, Papaccio G. Three years after transplants in human mandibles, histological and in-line holotomography revealed that stem cells regenerated a compact rather than a spongy bone: biological and clinical implications. Stem Cells Transl Med. 2013 Apr;2(4):316-24. doi: 10.5966/sctm.2012-0136.

18. Pirozzi G, Tirino V, Camerlingo R, La Rocca A, Martucci N, Scognamiglio G, Franco R, Cantile M, Normanno N, Rocco G. Prognostic value of cancer stem cells, epithelialmesenchymal transition and circulating tumor cells in lung cancer. Oncol Rep. 2013 May;29(5):1763-8. doi: 10.3892/or.2013.2294.
19. Desiderio V, De Francesco F, Schiraldi C, De Rosa A, La Gatta A, Paino F, d'Aquino R, Ferraro GA, Tirino V, Papaccio G. Human Ng2+ adipose stem cells loaded in vivo on a new crosslinked hyaluronic acid-Lys scaffold fabricate a skeletal muscle tissue. J Cell Physiol. 2013 Aug;228(8):1762-73. doi: 10.1002/jcp.24336.
20. Schiraldi C, Stellavato A, D'Agostino A, Tirino V, d'Aquino R, Woloszyk A, De Rosa A, Laino L, Papaccio G, Mitsiadis TA. Fighting for territories: time-lapse analysis of dental pulp and dental follicle stem cells in co-culture reveals specific migratory capabilities. Eur Cell Mater. 2012 Nov 23;24:426-40.
21. Ferraro GA, De Francesco F, Nicoletti G, Paino F, Desiderio V, Tirino V, D'Andrea F. Human adipose CD34+ CD90+ stem cells and collagen scaffold constructs grafted in vivo fabricate loose connective and adipose tissues. J Cell Biochem. 2013 May;114(5):1039-49. doi:10.1002/jcb.24443.
22. Tirino V, Desiderio V, Paino F, De Rosa A, Papaccio F, La Noce M, Laino L, De Francesco F, Papaccio G. Cancer stem cells in solid tumors: an overview and new approaches for their isolation and characterization. FASEB J. 2013 Jan;27(1):13-24. doi: 10.1096/fj.12-218222.
23. Rocco A, Compare D, Liguori E, Cianflone A, Pirozzi G, Tirino V, Bertoni A, Santoriello M, Garbi C, D'Armiento M, Staibano S, Nardone G. MDR1-P-glycoprotein behaves as an oncofetal protein that promotes cell survival in gastric cancer cells. Lab Invest. 2012 Oct;92(10):1407-18. doi: 10.1038/labinvest.2012.100.
24. Piepoli A, Mazzoccoli G, Panza A, Tirino V, Biscaglia G, Gentile A, Valvano MR, Clemente C, Desiderio V, Papaccio G, Bisceglia M, Andriulli A. A unifying working hypothesis for juvenile polyposis syndrome and Menetrier's disease: specific localization or concomitant occurrence of a separate entity? Dig Liver Dis. 2012 Nov;44(11):952-6. doi: 10.1016/j.dld.2012.05.019.
25. Tirino V, Desiderio V, Paino F, Papaccio G, De Rosa M. Methods for cancer stem cell detection and isolation. Methods Mol Biol. 2012;879:513-29. doi: 10.1007/978-1-61779-815-3_32.
26. Tirino V, Paino F, De Rosa A, Papaccio G. Identification, isolation, characterization, and banking of human dental pulp stem cells. Methods Mol Biol. 2012;879:443-63. doi: 10.1007/978-1-61779-815-3_26.
27. Tirino V, Papaccio G. A New, Most Likely Unusual Approach is Crucial and Upcoming for the Use of Stem Cells in Regenerative Medicine. Front Physiol. 2012 Jan 5;2:119. doi:10.3389/fphys.2011.00119. eCollection 2011.
28. Buommino E, Tirino V, De Filippis A, Silvestri F, Nicoletti R, Ciavatta ML, Pirozzi G, Tufano MA. 3-O-methylfunicone, from Penicillium pinophilum, is a selective inhibitor of breast cancer stem cells. Cell Prolif. 2011 Oct;44(5):401-9. doi: 10.1111/j.1365-2184.2011.00766.x.
29. Rocco A, Liguori E, Pirozzi G, Tirino V, Compare D, Franco R, Tatangelo F, Palaia R, D'Armiento FP, Pollastrone G, Affuso A, Bottazzi EC, Masone S, Persico G, Nardone G. CD133 and CD44 cell surface markers do not identify cancer stem cells in primary human gastric tumors. J Cell Physiol. 2012 Jun;227(6):2686-93. doi: 10.1002/jcp.23013.
30. Pirozzi G, Tirino V, Camerlingo R, Franco R, La Rocca A, Liguori E, Martucci N, Paino F, Normanno N, Rocco G. Epithelial to mesenchymal transition by TGFβ-1 induction increases stemness characteristics in primary non small cell lung cancer cell line. PLoS One. 2011;6(6):e21548. doi: 10.1371/journal.pone.0021548.
31. Mangano C, Paino F, d'Aquino R, De Rosa A, Iezzi G, Piattelli A, Laino L, Mitsiadis T, Desiderio V, Mangano F, Papaccio G, Tirino V. Human dental pulp stem cells hook into biocoral scaffold forming an engineered biocomplex. PLoS One. 2011 Apr 11;6(4):e18721. doi: 10.1371/journal.pone.0018721.
32. d'Aquino R, Tirino V, Desiderio V, Studer M, De Angelis GC, Laino L, De Rosa A, Di Nucci D, Martino S, Paino F, Sampaolesi M, Papaccio G. Human neural crest-derived postnatal cells exhibit remarkable embryonic attributes either in vitro or in vivo. Eur Cell Mater. 2011 Mar 22;21:304-16.
33. Tirino V, Desiderio V, Paino F, De Rosa A, Papaccio F, Fazioli F, Pirozzi G, Papaccio G. Human primary bone sarcomas contain CD133+ cancer stem cells displaying high tumorigenicity in vivo. FASEB J. 2011 Jun;25(6):2022-30. doi: 10.1096/fj.10-179036. Epub 2011 Mar 8.
34. Tirino V, Paino F, d'Aquino R, Desiderio V, De Rosa A, Papaccio G. Methods for the identification, characterization and banking of human DPSCs: current strategies and perspectives. Stem Cell Rev. 2011 Sep;7(3):608-15. doi: 10.1007/s12015-011-9235-9.
35. Ferraro GA, De Francesco F, Tirino V, Cataldo C, Rossano F, Nicoletti G, D'Andrea F. Effects of a new centrifugation method on adipose cell viability for autologous fat grafting. Aesthetic Plast Surg. 2011 Jun;35(3):341-8. doi: 10.1007/s00266-010-9613-8.
36. Paino F, Ricci G, De Rosa A, D'Aquino R, Laino L, Pirozzi G, Tirino V, Papaccio G. Ectomesenchymal stem cells from dental pulp are committed to differentiate into active melanocytes. Eur Cell Mater. 2010 Oct 7;20:295-305.
37. De Rosa A, Tirino V, Paino F, Tartaglione A, Mitsiadis T, Feki A, d'Aquino R, Laino L, Colacurci N, Papaccio G. Amniotic fluid-derived mesenchymal stem cells lead to bone differentiation when cocultured with dental pulp stem cells. Tissue Eng Part A. 2011 Mar;17(5-6):645-53. doi: 10.1089/ten.TEA.2010.0340.
38. Camerlingo R, Franco R, Tirino V, Cantile M, Rocchi M, La Rocca A, Martucci N, Botti G, Rocco G, Pirozzi G. Establishment and phenotypic characterization of the first human pulmonary blastoma cell line. Lung Cancer. 2011 Apr;72(1):23-31. doi: 10.1016/j.lungcan.2010.07.009. Epub 2010 Aug 17.
39. Mangano C, De Rosa A, Desiderio V, d'Aquino R, Piattelli A, De Francesco F, Tirino V, Mangano F, Papaccio G. The osteoblastic differentiation of dental pulp stem cells and bone formation on different titanium surface textures. Biomaterials. 2010 May;31(13):3543-51. doi: 10.1016/j.biomaterials.2010.01.056.
40. d'Aquino R, De Rosa A, Lanza V, Tirino V, Laino L, Graziano A, Desiderio V, Laino G, Papaccio G. Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. Eur Cell Mater. 2009 Nov 12;18:75-83.
41. De Francesco F, Tirino V, Desiderio V, Ferraro G, D'Andrea F, Giuliano M, Libondi G, Pirozzi G, De Rosa A, Papaccio G. Human CD34/CD90 ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. PLoS One. 2009 Aug 6;4(8):e6537. doi: 10.1371/journal.pone.0006537.
42. Tirino V, Camerlingo R, Franco R, Malanga D, La Rocca A, Viglietto G, Rocco G, Pirozzi G. The role of CD133 in the identification and characterisation of tumour-initiating cells in non-small-cell lung cancer. Eur J Cardiothorac Surg. 2009 Sep;36(3):446-53. doi: 10.1016/j.ejcts.2009.03.063. Epub 2009 May 22.
43. De Rosa A, De Francesco F, Tirino V, Ferraro GA, Desiderio V, Paino F, Pirozzi G, D'Andrea F, Papaccio G. A new method for cryopreserving adipose-derived stem cells: an attractive and suitable large-scale and long-term cell banking technology. Tissue Eng Part C Methods. 2009 Dec;15(4):659-67. doi: 10.1089/ten.TEC.2008.0674.
44. Costantino E, Maddalena F, Calise S, Piscazzi A, Tirino V, Fersini A, Ambrosi A, Neri V, Esposito F, Landriscina M. TRAP1, a novel mitochondrial chaperone responsible for multidrug resistance and protection from apoptotis in human colorectal carcinoma cells. Cancer Lett. 2009 Jun 28;279(1):39-46. doi: 10.1016/j.canlet.2009.01.018.
45. Di Fiore R, Santulli A, Ferrante RD, Giuliano M, De Blasio A, Messina C, Pirozzi G, Tirino V, Tesoriere G, Vento R. Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment. J Cell Physiol. 2009 May;219(2):301-13. doi: 10.1002/jcp.21667.
46. d'Aquino R, De Rosa A, Laino G, Caruso F, Guida L, Rullo R, Checchi V, Laino L, Tirino V, Papaccio G. Human dental pulp stem cells: from biology to clinical applications. J Exp Zool B Mol Dev Evol. 2009 Jul 15;312B(5):408-15.
47. Tirino V, Desiderio V, d'Aquino R, De Francesco F, Pirozzi G, Graziano A, Galderisi U, Cavaliere C, De Rosa A, Papaccio G, Giordano A. Detection and characterization of CD133+ cancer stem cells in human solid tumours. PLoS One. 2008;3(10):e3469. doi: 10.1371/journal.pone.0003469.
48. K, De Chiara A, Fazioli F, Longanesi-Cattani I, Cantelmo AR, Tirino V, Apice G, Rocco G, Lombardi ML, Carriero MV. Cell invasiveness in sarcomas: a possibly useful clinical correlation. Tumori. 2008 Jul-Aug;94(4):505-10.
49. D'Andrea F, De Francesco F, Ferraro GA, Desiderio V, Tirino V, De Rosa A, Papaccio G. Large-scale production of human adipose tissue from stem cells: a new tool for regenerative medicine and tissue banking. Tissue Eng Part C Methods. 2008 Sep;14(3):233-42. doi: 10.1089/ten.tec.2008.0108.
50. Malanga D, Scrima M, De Marco C, Fabiani F, De Rosa N, De Gisi S, Malara N, Savino R, Rocco G, Chiappetta G, Franco R, Tirino V, Pirozzi G, Viglietto G. Activating E17K mutation in the gene encoding the protein kinase AKT1 in a subset of squamous cell carcinoma of the lung. Cell Cycle. 2008 Mar 1;7(5):665-9.
51. Graziano A, d'Aquino R, Tirino V, Desiderio V, Rossi A, Pirozzi G. The stem cell hypothesis in head and neck cancer. J Cell Biochem. 2008 Feb 1;103(2):408-12