Education

Biography

Dr. Bryan Krantz is currently an associate professor in the Department of Microbial Pathogenesis, University of Maryland. Previously, he worked as an assistant professor in the Department of Molecular & Cell Biology, University of California (2006-14). He worked as a post-doctoral fellow with R. John Collier in the Department of Microbiology and Molecular Genetics, Harvard Medical School (2003-2006). He completed his PhD at Department of Biochemistry & Molecular Biology, University of Chicago (1997-2002). He received many honors in his career and published various articles in international journals.

Research Interest

Professional Activities:

Professional Experience

Prior Research Experience

• Harvard Medical School (2003-2006) Advisor: John Collier
• University of Chicago (1997-2003) Advisor: Tobin Sosnick
• Emory University (1994-1997) Advisor: Keith Wilkinson

Invited Seminars

1. “Molecular mechanisms of anthrax toxin translocation and trafficking.” University of Massachusetts, Amherst. Amherst, MA. 2014-Sep-25.
2. “Molecular mechanisms of anthrax toxin translocation and trafficking.” Purdue University. West Lafayette, IN. 2014-Jan-25.
3. “Molecular mechanisms of anthrax toxin translocation and trafficking.” Worchester Polytechnic Institute. Worchester, MA. 2013-Dec-9.
4. “Molecular mechanisms of anthrax toxin translocation and trafficking.” University of Maryland-Baltimore School of Medicine. Baltimore, MD. 2013-Nov-11.
5. “Molecular mechanisms of anthrax toxin translocation and trafficking.” The United States Army Medical Research Institute for Infectious Diseases (USAMRIID). Frederick, MD. 2013-Oct-1.
6. “Molecular mechanisms of anthrax toxin translocation and trafficking.” University of Maryland-Baltimore School of Dentistry. Baltimore, MD. 2013-Sept-30.
7. “Anthrax toxin protective antigen promotes lethal factor unfolding and translocation using an α-helical clamp.” Bacillus ACT 2013. Victoria, British Columbia, Canada. 2013-Sept-1-5.
8. “Molecular insights on anthrax toxin translocation and trafficking” University of Chicago Department of Microbiology. Chicago, IL. 2013-Aug-12.
9. “Anthrax toxin protective antigen promotes lethal factor unfolding and translocation using an α-helical clamp.” 2013 Madison Molecular Genetics of Bacteria & Phages Meeting. University of Wisconsin-Madison. Madison, WI. 2013-Aug-06-10.
10. “Anthrax toxin protective antigen promotes lethal factor unfolding and translocation using an α-helical clamp.” Selected as Young Investigator Speaker. 27th Annual Protein Society Meeting. Boston, MA. 2013-Jul-21.
11. “Anthrax toxin protective antigen promotes lethal factor unfolding and translocation using an α-helical clamp.” Southwest structural biology conference (SWSBC). University of Bristol, UK. 2013-July-2.
12. “Molecular insights on anthrax toxin translocation.” Oxford University, UK. 2013-July-1.
13. “Molecular insights on anthrax toxin translocation and trafficking” École Polytechnique Fédérale De Lausanne. Lausanne, Switzerland. 2013-June-27.
14. “Anthrax toxin protective antigen promotes lethal factor unfolding and translocation using an α-helical clamp.” European Workshop on Bacterial Protein Toxins (ETOX16). Universitätsklinikum Freiburg, Albert-Ludwigs-Universität Freiburg. Freiberg, Germany. 2013-June-25.
15. “Molecular insights on anthrax toxin translocation and trafficking” Tufts Medical School. Boston, MA. 2013-June-12
16. “Molecular Insights on Anthrax Toxin Unfolding and Translocation across Membranes” Membrane protein folding meeting. Biophysical Society. Seoul, South Korea. 2013-May-21.
17. “Molecular insights on anthrax toxin translocation and trafficking” National Institutes of Health. Bethesda, MD 2013-Feb-26.
18. “Molecular insights on anthrax toxin translocation and trafficking” Biosciences & Biotechnology Seminar. Lawrence Livermore National Laboratory. Livermore, CA. 2013-Jan-10.
19. “Molecular insights on anthrax toxin translocation and trafficking” Quantitative Biosciences Seminar. University of California, Berkeley. Berkeley, CA. 2012-Nov-19.
20. “Trafficking and transport: new molecular mechanisms of anthrax toxin regulation.” Gordon Research Conference. Microbial Toxins & Pathogenicity. Waterville Valley, NH. 2012-Jul-09.
21. “Anthrax toxin protein translocation powered by hydrogen+” Biophysical Society. San Diego, California. 2012-Feb-25.
22. “Insights on the molecular mechanism of transmembrane protein transport using anthrax toxin as a model system.” University of California, Los Angeles. 2012-Jan-17.
23. “Insights on the molecular mechanism of transmembrane protein transport using anthrax toxin as a model system.” University of Madison-Wisconsin. 2011-Dec-15.
24. “Insights on the molecular mechanism of transmembrane protein transport using anthrax toxin as a model system.” Northwestern University. 2011-Dec-08.
25. “Unfolding story of anthrax toxin protein translocation” Brandeis University. 2011-Nov-09.
26. “Unfolding story of anthrax toxin protein translocation.” New York University. 2011-Nov-08.
27. “Structural basis for the unfolding of anthrax lethal factor by protective antigen” Albert Einstein College of Medicine. Bronx, NY. 2010-Nov-04.
28. “Structural basis for the unfolding of anthrax lethal factor by protective antigen” University of Chicago. Chicago, IL. 2010-May-25.
29. “Structural basis for the pre-translocation unfolding of proteins by anthrax lethal toxin” University of California, Davis School of Medicine. Davis, CA. 2009-Dec-07.
30. “Structural Basis for the Pre-Translocation Unfolding of Proteins by Anthrax Lethal Toxin.” Children's Hospital Oakland Research Institute (CHORI). Oakland, CA. 2009-Oct-06.
31. “Anthrax toxin assembly & translocation: an interplay of form & function” Gordon Research Conference. Microbial Toxins & Pathogenicity. Proctor Academy, Andover, NH. 2008-Jul-13-18.
32. “Molecular mechanisms of anthrax toxin translocation” Bay Area Microbial Pathogenesis Symposium (BAMPS). University of California, San Francisco. 2008-Mar-29.
33. “How the anthrax toxin pore unfolds and then translocates its enzymatic factors across bilayer membranes.” Biopolymers Gordon Research Conference. Newport, RI. Jun 2006.
34. “Anthrax toxin translocation.” 50th Meeting of The Biophysical Society. Salt Lake City, UT. Feb 2006.
35. “Anthrax toxin's protective antigen pore: a protein translocase with a chaperone-like active site.” Harvard Medical School Dept. of Biological Chemistry & Molecular Pharmacology Seminar Series. Boston, MA. Oct 2005.
36. “Small molecules target protective antigen’s phenylalanine clamp—the site required to translocate lethal & edema factor into the host cell.” 2nd Annual Retreat of the New England Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research. Durham, NH. Sep 2005.
37. “The protective antigen binding domains of the lethal and edema factors of anthrax toxin unfold under acidic pH conditions.” 49th Meeting of The Biophysical Society. Long Beach, CA. Feb 2005.
38. “Realizing protein hydrogen bond network formation with kinetic D/H amide isotope effects.” University of Chicago. Chicago, IL. Dec 2001.
39. “When Do Hydrogen Bonds Form? D/H Backbone Amide Isotope Effects in Protein Folding Kinetics.” University of Chicago.Lake Geneva, WI. Oct 1998.

Publications

Peer-reviewed [PR]

1. Abrami L, Brandi L, Moayeri M, Brown MJ, Krantz BA, Leppla SH, van der Goot FG. (2013) “Hijacking Multivesicular Bodies Enables Long-Term and Exosome-Mediated Long-Distance Action of Anthrax Toxin.” Cell Rep. doi: pii: S2211-1247(13)00603-7. 10.1016/j.celrep. 2013.10.019.
2. Wynia-Smith SL, Brown MJ, Chirichella G, Krantz BA. (2012) “Electrostatic ratchet in the protective antigen channel promotes anthrax toxin translocation.” J. Biol. Chem. 287: 43753.
3. Kintzer AF, Tang II, Schawel AK, Brown MJ, Krantz BA. (2012) “Anthrax toxin protective antigen integrates poly-γ-D-glutamate and pH signals to sense the optimal environment for channel formation.” Proc. Natl Acad. Sci. 109: 18378.
4. von Moltke J, Trinidad NJ, Moayeri M, Kintzer AF, Wang SM, Rooijen N, Brown CR, Krantz BA, Leppla SH, Gronert K, Vance RE. (2012) "Rapid induction of lipid mediators is a novel effector function of the inflammasome in vivo" Nature. 490: 107.
5. Feld GK, Brown MJ, Krantz BA. (2012) "Ratcheting up protein translocation with anthrax toxin." Protein Sci. 21: 606.
6. Feld GK, Kintzer AF, Tang II, Thoren KL, Krantz BA. (2012) "Domain flexibility modulates the heterogeneous assembly mechanism of anthrax protective antigen." J. Mol. Biol. 415: 159.
7. Sterling HJ, Kintzer AF, Feld GK, Cassou CA, Krantz BA, Williams ER. (2012) "Supercharging protein complexes from aqueous solution disrupts their native conformations." J. Am. Soc. Mass. Spectrom. 23: 191.
8. Brown MJ, Thoren KL, Krantz BA. (2011) "Charge requirements for proton gradient-driven translocation of anthrax toxin." J. Biol. Chem. 286: 23189.
9. Thoren KL, Krantz BA. (2011) “The unfolding story of anthrax toxin translocation” Mol. Microbiol. 80: 588.
10. Sterling HJ, Cassou CA, Trnka MJ, Burlingame AL, Krantz BA, Williams ER. (2011) "The role of conformational flexibility on protein supercharging in native electrospray ionization" Phys. Chem. Chem. Phys. 13: 18288.
11. Sterling HJ, Daly MP, Feld GK, Thoren KL, Kintzer AF, Krantz BA, Williams ER. (2010) “Effects of supercharging reagents on noncovalent complex structure in electrospray ionization from aqueous solutions.” J. Am. Soc. Mass. Spectrom. 21: 1762.
12. Feld GF, Thoren KL, Kintzer AF, Sterling HJ, Tang II, Greenberg SG, Williams ER, Krantz BA. (2010) "Structural basis for the unfolding of anthrax lethal factor by the protective antigen oligomers." Nature Struct. Mol. Biol. 17:1383.
13. Kintzer AF, Sterling HJ, Williams ER, Krantz BA. (2010) "Anthrax toxin receptor drives protective antigen oligomerization and stabilizes the heptameric and octameric oligomer by a similar mechanism." PLoS ONE. 5(11):e13888.
14. Kintzer AF, Sterling HJ, Tang II, Abdul-Gader A, Miles AJ, Wallace BA, Williams ER, Krantz BA. (2010) "Role of the protective antigen octamer in the molecular mechanism of anthrax lethal toxin stabilization in plasma." J. Mol. Biol. 399: 741.
15. Thoren KL, Worden EJ, Yassif JM, Krantz BA. (2009) " Lethal factor unfolding is the most force-dependent step of anthrax toxin translocation." Proc. Natl Acad. Sci. 106: 21555.
16. Kintzer AF, Thoren KL, Sterling HJ, Dong KC, Feld GK, Tang II, Zhang TT, Williams ER, Berger JM, Krantz BA. (2009) "The protective antigen component of anthrax toxin forms functional octameric complexes." J. Mol. Biol. 292: 614.
17. Sosnick TR, Krantz BA, Dothager RS, Baxa M. (2006) "Characterizing the protein folding transition state using Ψ analysis." Chem. Rev. 106: 1862.
18. Krantz BA, Finkelstein A, Collier RJ. (2006) “Protein translocation through the anthrax toxin transmembrane pore is driven by a proton gradient.” J. Mol. Biol. 355: 968.
19. Christensen KA, Krantz BA, Collier RJ (2006). "The assembly and disassembly kinetics of anthrax toxin complexes." Biochemistry. 45: 2380.
20. Wolfe JT, Krantz BA, Rainey GJA, Young JAT, Collier RJ. (2005) “Whole-cell voltage clamp measurements of anthrax protective antigen pores.” J. Biol. Chem. 280: 39417.
21. Krantz BA, Melnyk RA, Zhang S, Juris SJ, Lacy DB, Wu Z, Finkelstein A, Collier RJ. (2005) “A phenylalanine clamp catalyzes protein translocation through the anthrax toxin pore.” Science. 309: 777.
22. Christensen KA, Krantz BA*, Melnyk RA, Collier RJ. (2004) “Interaction of the 20 kDa and 63 kDa fragments of anthrax protective antigen: kinetics and thermodynamics.” Biochemistry. 44: 1047. *Contributed equally to this work.
23. Krantz BA, Trivedi AD, Cunningham K, Christensen KA, Collier RJ. (2004) “Anthrax toxin’s lethal and edema factors unfold under acidic pH conditions.” J. Mol. Biol. 344: 739.
24. Sosnick TR, Dothager RS, Krantz BA. (2004) “Differences in the folding transition state of ubiquitin indicated by ϕ- and -analyses.” Proc. Natl Acad. Sci. 101: 17377.
25. Wigelsworth DJ, Krantz BA*, Christensen KA, Lacy DB, Juris SJ, Collier RJ. (2004) “Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen.” J. Biol. Chem. 279: 23349. *Contributed equally to this work.
26. Pimental RL, Christensen KA, Krantz BA, Collier RJ. (2004) “Anthrax toxin complexes: heptameric protective antigen can bind lethal factor and edema factor simultaneously.” Biochem. Biophys. Res. Comm. 322: 258.
27. Jacob J, Krantz B, Dothager RS, Thiyagarajan P, Sosnick TR. (2004) “Early collapse is not an obligate step in protein folding.” J. Mol. Biol. 338: 369.
28. Krantz BA, Dothager R, Sosnick TR. (2004) “Discerning the structure and energy of multiple transition states in protein folding using -analysis.” J. Mol. Biol. 337: 463.
29. Krantz BA, Mayne L, Rumbley J, Englander SW, and Tobin R. Sosnick. (2002) “Fast and slow intermediate accumulation and the initial barrier mechanism in protein folding.” J. Mol. Biol. 324: 359.
30. Krantz BA, Srivastava AK, Nauli S, Baker D, Sauer RT, Sosnick TR. (2002) “Understanding protein hydrogen bond network formation with kinetic D/H amide isotope effects.” Nature Struct. Biol. 9: 458.
31. Shi Z, Krantz BA, Kallenbach N, Sosnick TR. (2002) “Contribution of hydrogen bonding to protein stability estimated from isotope effects.” Biochemistry. 41: 2120.
32. Krantz BA, Sosnick TR. (2001) “Engineered metal binding sites map the heterogeneous folding landscape of a coiled coil.” Nature Struct. Biol. 8: 1042.
33. Krantz BA, and Sosnick TR. (2000) “Distinguishing between two-state and three-state models for ubiquitin folding.” Biochemistry. 39: 11696.
34. Yin L, Krantz B, Russell NS, Deshpande S, Wilkinson KD. (2000) “Nonhydrolyzable diubiquitin analogues are inhibitors of ubiquitin conjugation and deconjugation.” Biochemistry. 39:10001.
35. Krantz BA, Moran LB, Kentsis A, Sosnick TR. (2000) “D/H amide isotope effects reveal when hydrogen bonds form during protein folding.” Nature Struct. Biol. 7: 62.
36. Larsen CN, Krantz BA, Wilkinson KD. (1998) “Substrate specificity of deubiquitinating enzymes: ubiquitin C-terminal hydrolases.” Biochemistry. 37: 3358.
37. Amerik A, Swaminathan S, Krantz BA, Wilkinson KD, Hochstrasser M. (1997) “In vivo disassembly of free polyubiquitin chains by yeast Ubp14 modulates rates of protein degradation by the proteasome.” EMBO J. 16: 4826.

Non-peer-reviewed[NPR]

1. [NPR 2] Pandit AD, Krantz BA, Dothager RS, Sosnick TR. (2007) "Characterizing protein folding transition States using Ψ-analysis." Methods Mol. Biol. 350: 83.
2. [NPR 1] Krantz BA. (2002) Protein Folding: New Methods Unveil Rate-limiting Structures. Ph.D. diss., U. of Chicago.

Patents

1. Krantz BA, Kintzer AF, von Moltke JH. (filed Jan. 10, 2012; recordation Aug. 15, 2012; refiled Jan. 20, 2013) "Poly-Glutamic Acid Anti-Anthrax Composition and Methods for Using the Same" US Application Serial Nos. 61/585,183 and 13/738,911.