Professional Activities

Visiting Student Research Rotations

2002 (2 months):        Laboratory of Dr. Isabel Feito, Regional Service of Agroalimentarian Development (SERIDA) Research,
                                Villaviciosa, Asturias, Spain.
2005 (3 months):        Laboratory of Dr. Aixa Morales, Cajal Institute, Madrid, Spain
2005 to 2008 (3 months): Laboratory of Dr. Virginio García Martínez, Extremadura University, Badajoz, Spain.
2007 (1 month):         Laboratory of Dr. Amelia Aránega, Jaen University, Jaen, Spain
2007 (4 months):       Laboratory of Dr. Deborah Henderson, International Centre for Life, Newcastle Upon Tyne, UK.

Professional Societies Membership

2005-present       Member of the Spanish Cell Biology Society.


November 2013- November 2015    Connecticut Stem Cells Seed Grant: Cell Therapy     With ISL1+ Cardiac Progenitor Cells For Cardiac Repair After Myocardial Infarction (13-SCA-YALE-32). Principal Investigator: Oscar Bartulos.

Congress contribution

  • XI Congress of the Cell Biology Spanish Society. Oral Presentation (2005) Cádiz, Spain.
  • V Congress of the Development Biology Spanish Society. Poster (2006) Alicante,Spain.
  • Joint Meeting of the British and Spanish Developmental Biology Societies. Poster (2008) Sevilla, Spain.
  • Workshop in Live Imaging at Yale University (April 2012). Co-organizer in collaboration   with Perkin Elmer Company.
  • Patents

    “Use of catecholamines in stem cell differentiation to cardiomyocytes”
    Spain: ES P200802422 (2008-8-13). WO 2010/018297 A1.  Inventors: Catalina Hernández Sánchez, Óscar Bártulos Encinas, Flora de Pablo Dávila and Amelia Aránega Jiménez

    Research Fellowships

    Biological Research Centre (CIB), Madrid, Spain      02/01/2004 - 04/30/2004
    In vitro fecundation (FIV- Madrid).

    Biological Research Centre (CIB), Madrid, Spain     06/01/2004 - 12/31/2004
    Health Institute Carlos III.

    Biological Research Centre (CIB), Madrid, Spain     04/01/2005 to 03/31/2009
    Spanish Education and Science Ministery (MEC)

    Laboratory Experience

    Molecular Biology

    • Protein and RNA immunoprecipitation
    • RNA pull-down
    • Chromatin Immunoprecipitation (ChIP)
    • High throughput Sequencing and Crosslinking Immunoprecipitation (HITS-CLIP)
    • RT-PCR  and qRT-PCR
    • Cloning
    • DNA, RNA and protein extraction
    • Southern Blot, Northern Blot and Western Blot
    • ELISA assay
    • TUNEL assay (TdT-mediated dUTP nick end labelling)
    • Beta elimination
    • In situ hybridization: colour and fluorescence in whole mount and tissue sections.
    • Immunohistochemistry: colour and fluorescence in whole mount and tissue sections

    Cell culture and embryo manipulation

    • Human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cell (iPSC) culture and differentiation: H1, H7, H9, R1, E14Tg2a.IV, E7, AB2.2.
    • Primary cell culture: human smooth muscle cells and fibroblasts, mouse splenocytes and CD4+ cells.
    • Cell line culture and transient transfection
    • Chicken embryo manipulation and culture: New culture, EC culture and minimal medium
    • Chicken embryo electroporation:  somite electroporation “in ovo” in HH6-HH7 stage heart electroporation in EC culture in HH3-HH4 stage
    • Microbeads implantation in chicken embryo:
    • HH4: in Germinal Crescent Crest and lateral to Hensen´s node
      HH5: lateral and inside Heart formation region
      HH7, HH8- and HH8: lateral to endocardial tubes

    Other Techniques

    • Flow cytometry
    • Magnetic beads (MACS) separation
    • Mouse colony maintenance / embryo manipulation
    • Zebrafish colony maintenance / embryo manipulation

    Oscar Bartulos Encinas

    Associate Research Scientist


    • : 203-737-3431


      Cardiovascular Division Internal Medicine
      Yale University