Education

Biography

Dr. Olga Margarita Pulidois a medical doctor (MD) from the Central University of Caracas, Venezuela, with a postgraduate medical training at the University of Miami, USA, a Certificate from the Educational Council for Foreign Medical Graduates, USA; F.L.E.X. Medical Licensing Examination, State of Illinois, USA; Certificate for Foreign Physicians from the Medical Council of Canada, and professional registration with the College of Physicians of Nova Scotia, Canada and the State of Illinois, USA.

Dr. Pulidotrainedin Pathology at Queen's University, Canada, obtaining a Master of Sciencedegree,and a certification as Medical Specialist in Anatomical Pathology by the American Board of Pathology, USA. Further training includes: Neuropathology at theUniversity of Western Ontario, CA; Post-Doctoral Fellowship in Neuroscience, McMaster University, Hamilton, ON, CA.; certification as ToxicologicPathologist “Fellow of the International Academy of Toxicologic Pathology
Dr. Pulido is an Adjunct Professor at the Department of Pathology and Laboratory Medicine, University of Ottawa
Her professional experience encompasses: Hospital Medical Staff, University Faculty Member, and 26 years as Medical Specialist at Health Canada, Government of Canada, including 21 years as Head of Pathology, Toxicology Research and Senior Medical Advisor, Food Directorate, Health Products and Food Branch. Her commitment with health sciences/medical education, and biomedical research include numerous publications in peer reviewed journals, book chapters, book editing, journal editorial boards, reviewer, international teaching and lecturing invitations e.g.: former invited professor at the Doctorate Program in Neurotoxicology and Psychopharmacology, University of RoviraiVirgili, Reus, Spain.

Dr. Pulido is an active volunteer within professional organizations and the community in a variety of roles including:organization of continuing education programs, public education forums and consumers organizations e.g.: Canadian, Celiac Association; health commentaries at a local Hispanic Community TV program. Currently act as: Alumni Career Mentor for Graduate Students, School of Graduate Studies, Queen's University, Kingston, Ontario, and Member of the Constituency Council Advisory Group,Centre for Addiction and Mental Health (CAMH), Toronto, Ontario. Her research interest focuses on: toxicologic pathology, food safety, environmental contaminants, glutamate receptors, food allergies and intolerances: celiac disease and gluten related disorders.

Research Interest

• Toxicologic Pathology
• Glutamate Receptors
• Phycotoxins
• Neurotoxicology
• Food allergy and intolerances
• Celiac Disease and gluten related conditions

Professional Activities:

Publications

1. Pulido Olga M. “Letter to the Editor GHFBB; Response to Peña AS: What Is the Best Histopathological Classification for Celiac Disease? Does It Matter?”.Gastroenterology and Hepatology From Bed to Bench  (2016)9.1: 68–69.
2. La Vieille S, Pulido OM, Abbott M, Koerner TB, Godefroy S. Celiac disease and gluten-free oats: A Canadian position based on a literature review. Can J Gastroenterol Hepatol. 2015 Sep 30. pii: 17129.
3. Pulido OM.Marine Algal Toxins: Seafood Safety, Human Health and Beyond. Oceanography 2014, 2: e111. doi:10.4172/2332-2632.1000e111 (by Invitation)
4. Olga M Pulido. Chapter 8. Domoic Acid: Biological Effects and Health Implications. In Rossini, Gian Paolo, ed. Toxins and Biologically Active Compounds from Microalgae. Vol. 2. Biologicl Effects and Risk Management. Section 2. Impact of Toxic Microalgae and Microalgal Toxins on Biological Systems. CRC Press, 2014, pp 219-252 (by Invitation)
5. Pulido O, Zarkadas M, Dubois S, Macisaac K, Cantin I, La Vieille S, Godefroy S, Rashid M. Clinical features and symptom recovery on a gluten-free diet inCanadian adults with celiac disease. Can J Gastroenterol. 2013 Aug;27(8):449-53. sss
6. Olga M. Pulido and Santokh Gill. Chapter 35, Food and Toxicologic Pathology: An Introduction.Haschek and Rousseaux’s Eds. Handbook of Toxicologic Pathology 3e,VOLUME II – Safety Assessment including Current and Emerging Issues in Toxicologic Pathology Part 3 – Food and the Environment: Selected Toxic Agents.  Elsevier, 2013. pp 1051-1073 (By invitation)
7. S. Gill, W. Bowers, J. Nakai, I. Chu, D. Moir, A.Yagminas, R. Mueller and O. Pulido. Effects of Environmentally Relevant Mixtures of Persistent Organic Pollutants on the Developmental Neurobiology in Rats. Toxicol Pathol 41:38-47,2013
8. Marion Zarkadas , Sheila Dubois , Krista MacIsaac , Isabelle Cantin, Mohsin Rashid, Karen C. Roberts, Sébastien La Vieille, Samuel Godefroy, Olga M. Pulido Living With Celiac Disease and A Gluten-Free Diet: A Canadian Perspective. J Hum Nutr Diet. 28:10-23,2012.
9. Zoë Gillespie, Olga Pulido and Elizabeth Vavasour. Risk Assessment Approaches for Carcinogenic Food Contaminants.  International Food Risk Analysis Journal Volume 1, Issue 1, Sep 2011
10. Pulido Olga M. Chapter 1. Immune-mediated adverse reactions to dietary proteins. In Allergen Management in the Food Industry, Editors: Joyce I. Boye and Samuel Godefroy; John Wiley & Sons Publishing Co. 2010, pp 3-32. (By invitation)
11. Pulido Olga M., Gillespie Z, and Godefroy SB. Chapter 4. The Canadian criteria for the establishment of new priority food allergens: evidences for the inclusion of mustard and insufficient evidences for garlic and onion as priority allergens in Canada. In Allergen Management in the Food Industry, Editors: Joyce I. Boye and Samuel Godefroy; John Wiley & Sons Publishing Co. 2010, pp 75-130. (By Invitation)
12. Pulido Olga M. , Gillespie Z, Zarkadas M, Dubois S, Vavasour E, Rashid M, Switzer C, Godefroy SB. Chapter 6. Introduction of oats in the diet of individuals with Celiac disease. A systematic review. Adv Food Nutr Res. 2009; 57:235-85 (By invitation).
13. Pulido OM. Domoic acid toxicologic pathology: a review. Marine Drugs. 2008 May 28; 6(2): 180-219.
14. Gill SS, Hou Y, Ghane T, Pulido OM. Regional susceptibility to domoic acid in primary astrocyte cells cultured from the brain stem and hippocampus. Marine Drugs. 2008 Feb 14; 6(1):25-38.
15. Gill S, Barker M, Pulido O. Neuroexcitatory targets in the female reproductive system of the nonhuman primate (Macaca fascicularis). Toxicol Pathol. 2008; 36(3):478-84.
16. Chu I, Bowers WJ, Caldwell D, Nakai J, Wade MG, Yagminas A, Li N, Moir D, El Abbas L, Håkansson H, Gill S, Mueller R, Pulido O.  Toxicological effects of in utero and lactational exposure of rats to a mixture of environmental contaminants detected in Canadian Arctic human populations. J Toxicol Environ Health A. Jan; 71(2):93-108, 2008.
17. Gill S, Veinot J, Kavanagh M, Pulido O.  Human heart glutamate receptors – implicationsfor toxicology, food safety, and drug discovery. Toxicol Pathol. 35(3):411-7, 2007.
18. Rashid M, Butzner D, Burrows V, Zarkadas M, Case S, Molloy M, Warren R, Pulido O, Switzer C.  Consumption of pure oats by individuals with celiac disease: a position statement by the Canadian Celiac Association. Can J Gastroenterology. Oct; 21(10):649-51, 2007.
19. Morton D, Kemp RK, Francke-Carroll S, Jensen K, McCartney J, Monticello TM, Perry R, Pulido O, Roome N, Schafer K, Sellers R, Snyder PW.  Best practices for reporting pathology interpretations within GLP toxicology studies. Toxicol Pathol.; 34(6):806-9, 2006.
20. Ih Chu, Wayne J. Bowers, Don Caldwell, Jamie Nakai, Olga Pulido, Al Yagminas, Michael G. Wade, David Moir, Santokh Gill, and Rudi Mueller. Toxicological Effects of Gestational and Lactational Exposure to a Mixture of Persistent Organochlorines in Rats: Systemic Effects.  Toxicol. Sci. 88: 645-655, 2005.
21. Santokh Gill and Olga Pulido. Distribution of Glutamate Receptors in Peripheral Tissues. In Glutamate Receptors in Peripheral Tissues, S.Gill &O. Pulido (Eds), Kluwer Academic Plenum Press, NY, page 3-26, 2005
22. Olga Pulido, Don Caldwell, Meghan Murphy and Santokh Gill. Chapter 15. Practical Considerations in Assessing Immunhistopathology in Rodents and Nonhuman Primates. In Investigative Immunotoxicology, Helen Tryphonas et al (Eds), Tylor & Francis International, CRC press, USA, p 229, 2005.
23. Cooke G.M., Tryphonas H., Pulido O., Caldwell. D., Bondy G.S., Forsyth D. In utero and postnatal oral (gavage) exposure of Sprague-Dawley rats to low doses of tributyltin chloride. Part 1: Toxicology, Histopathology and Clinical Chemistry. Food and Chemical Toxicology Feb; 42(2):211-20, 2004
24. H. Tryphonas, G. Cooke, D. Caldwell, G. Bondy, M. Parenteau S. Hayward and O. Pulido.  Oral (gavage), in utero and post-natal exposure of Sprague-Dawley rats to low doses of tributyltin chloride.  Part II: Effects on the immune system. Food and Chemical Toxicology Feb; 42(2):221-35.2004.
25. WJ Bowers, JS. Nakai, I Chu, M Wade, D Moir, S Gill, R Mueller, O Pulido. Early developmental neurotoxicity of a PCB/organochlorine mixture in rodents after gestational and lactational exposure. Toxicol Sci.; 77(1):51-62. 2004.
26. Bowers, W.J., Nakai, J.S., Chu, I., Wade, M., Moir, D., Gill, S., Mueller, R., Pulido, O.Neurobehavioral toxicity of in utero and lactational exposure to a chemical mixture based on blood levels in Canadian Arctic populations. Neurotoxicology and Teratology, 25:395, 2003
27. Gill, S., Murphy, M., Clausen, J., Richard, D., Quilliam, M. MacKinnon, S., LeBlanc, P. &Pulido O. Neural Injury Biomarkers of Novel Shellfish Toxins, Spirolides. A pilot study using immunochemical and transcriptional Analysis.  Neurotoxicology 34: 593-604, 2003.
28. R.Mueller, S. Gill and Olga Pulido.  The monkey (Macaca fascicularis) heart neuralstructures and conducting system. An immunochemical study of selected neural biomarkers and glutamate receptors.  Toxicologic Pathology, 31: 1-8, 2003.
29. Wang, G. -S.; Karamchandani, J.;Pulido, O.; Rosenberg, L.; Scott, F.W. Altered islet homeostasis before classic insulinitis in BB rats. Diabetes Metab. 28, 3S90–3S97, 2002.
30. Bondy, G.; Barker, M.; Cooke, G.; Curran, I.; Doucet, J.; Gill, S.; Lok, E.; Mehta, R.; Pulido, O; Robertson, P.; Rowsell, P.; Norstrom, R; Moisey, J. In Synopsis of Research Conducted under the 2001-2002 Northern Contaminants Program, Kalhok, S., Ed.; Indian and Northern Affairs Canada, Ottawa, 2002.
31. O. Pulido.  Ochratoxin hepatotoxicity. Moderators Report. In Registry of Toxicologic Pathology for Animals (RTPA). Armed Forces Institute of Pathology (AFIP), Washington, D.C., 2002.
32. O. Pulido. Fumonisin B1 renal toxicity. Moderators Report. In Registry of Toxicologic Pathology for Animals (RTPA).  Armed Forces Institute of Pathology (AFIP), Washington, D.C, 2002.
33. O.Pulido. Retinal Degeneration in Rats and Dogs associated to the oral administration of ATP-sensitive potassium channel activators. Moderators Report. In Registry of Toxicologic Pathology for Animals (RTPA).  Armed Forces Institute of Pathology (AFIP), Washington, D.C, 2002.
34. O.Pulido. Myopathy related to the administration of HMG-CoA reductase inhibitor. Moderators Report. In Registry of Toxicologic Pathology for Animals (RTPA). Armed Forces Institute of Pathology (AFIP), Washington, D.C., 2002.
35. Olga Pulido, Don Richard, Michael Quilliam, Joann Clausen, Meghan Murphy, Peter Smyth, Ruedi Mueller and Santokh Gill. Toxicological Neuropathology from Domoic Acid to Spirolides. The Health Canada Experience. Can. J. Fisheries and Aquatic Sci. Proc. of the 7th Canadian Workshop on Harmful Marine Algae, 2001.
36. Bryce, F., Iverson, F., Andrews, P., Barker, M.,Cherry, W., Mueller, R., Pulido, O., S. Hayward, S.,Fernie, S. and Arnold, D.L. Effects elicited by toxaphene in the cynomolgus (M. fascicularis) monkey. A pilot study. Food Chemical Toxicology, 39:1243-1251, 2001.
37. Gill SS, Pulido OM.  Glutamate receptors in peripheral tissues: current knowledge, future research, and implications for toxicology. Toxicol Pathol. 2001 Mar-Apr; 29(2):208-23. Review.
38. Gill, S., Mueller, R., McGuire, P. andPulido, O. Potential target sites in peripheral tissues for excitatory neurotransmission and excitotoxicity. Toxicologic Pathology 28:277-284, 2000.
39. Pulido, O., Mueller, R., Smyth, P., Clausen, J. and Gill, S. Heart and brain glutamate receptors- a common mechanism for excitotoxicity? Brain Pathology 10:681, 2000.           
40. Wang, G.-S., Gruber, H., Smyth, P.,Pulido, O., Rosenberg, L., Duguid, W. and Scott F.W. Hydrolysed casein diet protects diabetes-prone BB rats from developing diabetes by promoting islet neogenesis. Journal of Autoimmunity 15:407-416, 2000.
41. Sarwar, G; M.R. L'Abbe, S.P.J. Brooks, E. Lok, G. Cooke, O. Pulido and P. Thibert. Dietary Phytoestrogens: Safety, Nutritional Quality and Health Considerations   in ACS book entitled "Quality Management of Nutraceuticals: edited by C-T. Ho and Q.Y. Zheng., 2000
42. Gill, S., Pulido, O., Mueller, R. and McGuire, P. Immunohistochemical characterization of metabotropic glutamate receptors in the rat heart. Brain Research Bulletin 48:143-146, 1999.
43. Pulido, O. Demonstration of glutamate receptors in body tissues and their relation to food safety and excitotoxicity. Soc. for Neuroscience Press Book, Part II, pg. 121, 1999.
44. Gill, S., Pulido, O., Mueller, R. and McGuire, P. Molecular and immunochemical characterization of ionotropic glutamate receptors in the rat heart. Brain Research Bulletin 46: 429-434, 1998.
45. J. Truelove, R. Mueller, O.Pulido, L. Martin, S. Fernie, and F. Iverson. 30- Day Oral Toxicity of Domoic Acid in Cynomolgu Monkeys: Lack of Overt Toxicity at Doses Approaching the Acute Toxic Dose. Natural Toxins 5: 111-114, 1997
46. J. Truelove, R. Mueller,O. Pulidoand F. Iverson. Subchronic toxicity Study of Domoic Acid in Rat.  Food Chem. Tox. 34: 525, 1996
47. Niles, L.P., Pulido,O.M. and Pickering, D.S.  Age-related changes in GABA and Benzodiazepine receptor binding in rat brain are influenced by sampling time.  Prog. Neuropsychopharmacol. & Biol. Psychiat. , 12: 337-344, 1988.
48. Pulido, O.:  Aging and a multicultural Society.  In "Proc. of Multicultural Health Workshop", Hamilton, Ontario, 1988, Pulido, O. and Niles, E. (Eds), Pine Crest.
49. Pulido, O.:  Biology of Human Aging. Monograph and Videotapes I-III,  General Concepts in Gerontology and Theories of Aging.  E. Brain (Ed).  McMaster University, Hamilton, 1988
50. Pulido, O. and Clifford, J.:  Age-associated changes in the circadian rhythm of retinal N-acetyl serotonin (NAS) and melatonin in rats with pigmented eyes.  Exp.  Gerontology 21:23-30, 1986.
51. Davis, D., Majumdar, B., Pulido, O.:  Report and Recommendation on The Impact of Transculturalism on Health and Health Care.  Proc. Transcultural Health Workshop, Faculty of Health Sciences, McMaster University, 1987.
52. Pulido, O.M., Clifford, J., Brown, C. and Cole, S.:  Pineal and extra-pineal N-acetyl serotonin (NAS) and melatonin as function of age.  International Workshop on the Pineal Gland during Development, Neuroendocrinology Lett. Vol. 7(4), 1985.
53. Niles, L.P. and Pulido, O.M.:  Age-related changes in GABA receptors.  International Workshop on the Pineal Gland during Development, Neuroendocrinology Lett. Vol. 7(4), 1985.
54. Brown, G.M.,Pulido, O., Grota, L.J. and Niles, L.P.:  N-acetyl-serotonin in the central nervous system.  Prog. Neuropsycho-pharmacol. and Biol. Psychiat.  8:475-480, 1984.
55. Brown, G.M., Niles, L.P., Pulido, O., Ho, A. and Grota, L.J.:  Extrapineal melatonin and N-acetyl serotonin.  PECS Proceedings, 1984.
56. Pulido, O., Brown, G.M. and Grota, L.J.:  B-adrenergic regulation of N-acetyl serotonin (NAS) synthesis in the rat cerebellum.  Life Sciences 33:1081, 1983.
57. Pulido, O., Brown, G.M. and Grota, L.J.:  An Immunohistochemical method for the localization of N-acetyl serotonin (NAS) in the central nervous system.  Description, validation and application of the technique.  J. Histochem. Cytochem.  31:1343, 1983.
58. Psarakis, S., Pulido, O., Brown, G.M., Grota, L.J. and Smith, G.K.:  Identification and quantitation of N-acetyl serotonin in the developing hippocampus of the rat by immunohistology.  Prog. Neuropsychopharmacol. and Biol. Psychiat.  6:439, 1982.
59. Pulido, O., Brown, G.M. and Grota, L.J.:  Localization of N-acetyl serotonin (NAS) in the rat hindbrain by immunohistology.  Prog. Neuropsychopharmacology 5:573, 1981.
60. Pulido, O., Brown, G.M. and Grota, L.J.:  Immunohistological localization of N-acetyl serotonin in the rat brain. J. Histochem. and Cytochem. 28:613, 1980.
61. Pulido, O.M., Bencosme, S.A., de Bold, M.L. and de Bold, A.:  Intracellular B-cell serotonin and the dynamics of insulin release.  Diabetologia 15:197-204, 1978
62. Pulido, O., de Bold, A., de Bold, M. and Bencosme, S.A.:   Demonstration of a tryptaminergic mechanism in the rat B-cell.   Experientia 33:268-270, 1977.
63. Gonzalez, J.E., Pulido, O. and Rojas, G.: Accion del SH 90 000 C (d-norgestrel) sobre la estructura endometrial humana.  In: “II Reunión Latino-americana SH 90 000 C.  México, 1970.  Eds. Scharff, H.J.:  Ruiz Albrecht F. Saladruck, Berlin, p. 104, 1972.
64. Potenza, Von, L.,Pulido, O. and Rojas, G.:  Zur wirkung won d-norgestral auf die struktur des menschilichem endometriums vergleichende underschug mit dem light und electronenmikroskop.  Medizinische metteilugen Schering 1:20, 1972.
65. Lejter, M., Gonzalez, J.E., Pulido, O. and Cardenas, N.:  Sindrome de Reye.  Arch. Venez. Pueric y Pedt.  2:31, 1972.
66. Pulido, O. and Contreras, J.:  Embarazo tubarico en pacientes con espiral intrauterino.  Bol. Med. Hosp. Centr. FF. AA. (Caracas) 2:7, 1971.